Cell-Based ELISA Kits

ELISA Genie Cell-Based ELISA Kits

The ELISA Genie Cell-Based ELISA Kits can be used for measuring the relative amounts of protein in cultured cells, as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on protein expression.

These in vitro immunohistochemistry ELISA assays are a premium range of convenient, lysate-free, high throughput tests that can monitor protein expression profile in cells.

Qualitative determination of target protein concentration is achieved via indirect ELISA.


Features

  • Excellent Sensitivity, Specificity, and Efficiency - ELISA Genie Cell-Based ELISA kits allow for the high throughput screening of several samples with a high signal to noise ratio.

  • Minimal manual labour - No lysate preparation or lysate transfer steps required.

  • Convenient - can be used to assess the effects of several different treatments (activators and inhibitors) on a wide range of cell lines.

  • Adaptable - Allows for the measurement of target analytes in situ in both adherent and suspension cells.

Contents

Fluorometric Cell-Based ELISA Kits

  • TBS
  • Quenching Buffer
  • Blocking Buffer
  • Wash Buffer
  • Primary Antibody Diluent
  • α-Target Antibody
  • α-GAPDH Antibody
  • Dye-1 Conjugated Anti-Rabbit IgG Antibody
  • Dye-2 Conjugated Anti-Mouse IgG Antibody
  • 96-Well Black Cell Culture Clear-Bottom Microplate
  • Adhesive Plate Seals

Colorimetric Cell-Based ELISA Kits

  • TBS
  • Quenching Buffer
  • Blocking Buffer
  • Wash Buffer
  • Primary Antibody Diluent
  • α-Target Antibody
  • α-GAPDH Antibody
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • SDS Solution
  • Crystal Violet Solution
  • Ready-to-use Substrate
  • Stop Solution
  • 96-Well Cell Culture Clear-Bottom Microplate
  • Adhesive Plate Seals

Principle

Fluorometric Cell-Based ELISA

For Fluorometric Cell-Based ELISA, the target protein is captured by target-specific primary (1°) antibodies while Dye 1-conjugated and Dye 2-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the dye conjugated to the 2° antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes.

Figure : Assay format of ELISA Genie Fluorometric Cell-Based ELISA Kit

Colorimetric Cell-Based ELISA

For Colorimetric Cell-Based ELISA, the target protein is captured by target-specific primary (1°) antibodies while the HRP-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the HRP enzyme conjugated to the 2° antibody can catalyze a colorimetric reaction upon substrate addition.

 

Figure : Assay format of ELISA Genie Colorimetric Cell-Based ELISA Kit


Key ELISA Genie Cell-Based ELISA Kits

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