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Anti-Avelumab (Bavencio®) ADA Qualitative ELISA Kit

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ELISA Kit Technical Manual

Anti-Avelumab (Bavencio®) ADA Qualitative ELISA Kit

Enzyme immunoassay for the qualitative determination of antibodies to avelumab in serum and plasma.The ELISA Genie® Antibody to avelumab (Bavencio®) Enzyme-Linked-ImmunoSorbent Assay (ELISA) Kit is intended for the qualitative determination of antibodies to avelumab (Bavencio®) in serum and plasma. It is for professional use only.

Product Information

ApplicationFree drug
Required Volume (μl)10
Total Time (min)140
Sample TypeSerum, Plasma
Number of Assays96
Detection Limit (ng/mL)plus/minus
Spike Recovery (%)-
Shelf Life (year)1

About Anti-Avelumab (Bavencio®) ADA Qualitative

Avelumab (Bavencio®) (also known as MSB0010718C) is an investigational fully human anti-PD-L1 IgG1 lambda monoclonal antibody that has a molecular weight of approximately 147 kDa. By inhibiting PD-L1 interactions, avelumab is thought to enable the activation of T-cells and the adaptive immune system. By retaining a native Fc-region, avelumab is thought to engage the innate immune system and may induce antibody-dependent cellmediated cytotoxicity (ADCC). Importantly, avelumab has not shown antibody-dependent cell-mediated cytotoxicity against immune cell subsets in humans.

Anti-Avelumab (Bavencio®) ADA Qualitative ELISA Kit test principle

The Elisa Genie® Antibody to avelumab (Bavencio®) ELISA is a sandwich assay for the determination of antibodies against avelumab in serum and plasma samples. During the first incubation period, antibodies to avelumab (ATAV) in patient serum/ plasma samples are captured by the drug avelumab (Bavencio®) coated on the wall of the microtiter wells. After washing away the unbound components from samples, a peroxidase-labelled specific conjugate is added to each well and then incubated. After a second washing step, the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The intensity of the reaction colour is directly proportional to the concentration of ATAV in sample.


BAVENCIO is a trademark of MERCK KGAA

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