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Aldosterone ELISA Kit

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SKU:
UNFI0050

Description

Aldosterone ELISA Kit - Information

The Aldosterone ELISA Kit can assay for Aldosterone in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Aldosterone ELISA Kits Work?

The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Aldosterone . During the reaction, Aldosterone in the sample or standard competes with a fixed amount of Aldosterone on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Aldosterone . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Aldosterone in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Aldosterone ELISA Kit - Data

Product name

ALD(Aldosterone) ELISA Kit

Catalogue No.

EU2580

Alias

ALD(Aldosterone)

Detection method

Competitive ELISA Coated with Antigen

Application

This immunoassay kit allows for the in vitro quantitative determination of ALD concentrations in serum plasma and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Storage

4 €žÆ’ for 6 months

Recovery

Matrices listed below were spiked with certain level of ALD and the recovery rates were calculated by comparing the measured value to the expected amount of ALD in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-104 96
EDTA plasma(n=5) 86-105 95
UFH plasma(n=5) 88-101 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 89-104% 86-102% 85-99%
EDTA plasma(n=5) 84-92% 84-97% 82-101% 87-99%
UFH plasma(n=5) 84-98% 80-91% 82-91% 87-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
General
ELISA Type:
Competitive
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