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Anti-Adalimumab (Humira®) ADA Qualitative ELISA Kit

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SKU:
HUMB00010
$1,022

Description

ELISA Kit Technical Manual

Anti-Adalimumab (Humira®) ADA Qualitative ELISA Kit

Enzyme immunoassay for the quantitative determination of antibodies to adalimumab in serum and plasma with confirmation. Adalimumab (Humira®) was associated to the development of anti-Adalimumab antibodies, even some were reported to be neutralizing, in various percentages of patients during therapy with the drug Humira®. This might lead to severe complications. The ELISA Genie Antibody to Adalimumab ELISA Kit can be efficiently used for monitoring anti-Adalimumab antibodies during therapy and offers the clinician a tool for decision on possible preventive measures such as possible addition of immunosuppressive drug to reduce anti-Adalimumab antibodies.

Product Information

ApplicationFree drug
Required Volume (μl)10
Total Time (min)140
Sample TypeSerum, Plasma
Number of Assays96
Detection Limit (ng/mL)30 (ng/mL)
Spike Recovery (%)85-115%
Shelf Life (year)1

About Anti-Adalimumab (Humira®) ADA Qualitative

Adalimumab (Humira®) is a tumor necrosis factor (TNF) blocker indicated for treatment of Rheumatoid Arthritis, Juvenile Idiopathic Arthritis, Psoriatic Arthritis, Ankylosing Spondylitis, Crohn’s Disease, Plaque Psoriasis. Accoring to the manufacturer’s product insert, the use of Adalimumab (Humira®) was associated to the development of anti-adalimumab antibodies, even some were reported to be neutralizing, in various percentages of patients during therapy with the drug Humira®. This might lead to severe complications. The Elisa Genie Antibody to Adalimumab ELISA Kit can be efficiently used for monitoring antiadalimumab etanercept antibodies during therapy and offers the clinician a tool for decision on possible preventive measures such as possible addition of immunosuppressive drug to reduce anti-adalimumab antibodies. With this Elisa Genie ELISA test, antibodies to adalimumab can be detected in patients receiving Humira®

Anti-Adalimumab (Humira®) ADA Qualitative ELISA Kit test principle

The Elisa Genie Antibody to Adalimumab (Humira®) ELISA is a sandwich assay for the determination of antibodies against adalimumab in serum and plasma samples. During the first incubation period, the drug adalimumab coated on the wall of the microtiter wells captures the antibodies to adalimumab in patient serum and plasma samples. After washing away the unbound components from samples, a Peroxidase-labelled conjugate is added to each well and then incubated. After a second washing step, the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The intensity of the reaction colour is directly proportional to the concentration of antibodies to Adlimumab in sample.

Trademarks

Humira® is a registered trademark of AbbVie Biotechnology, Inc.

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