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8-OHdG / 8-Hydroxydeoxyguanosine ELISA Kit

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SKU:
UNFI0029

Description

8-OHdG / 8-Hydroxydeoxyguanosine ELISA Kit - Information

The 8-OHdG / 8-Hydroxydeoxyguanosine ELISA Kit can assay for 8-OHdG / 8-Hydroxydeoxyguanosine in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our 8-OHdG / 8-Hydroxydeoxyguanosine ELISA Kits Work?

The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-OHdG / 8-Hydroxydeoxyguanosine . During the reaction, 8-OHdG / 8-Hydroxydeoxyguanosine in the sample or standard competes with a fixed amount of 8-OHdG / 8-Hydroxydeoxyguanosine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 8-OHdG / 8-Hydroxydeoxyguanosine . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 8-OHdG / 8-Hydroxydeoxyguanosine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

8-OHdG / 8-Hydroxydeoxyguanosine ELISA Kit - Data

Product name

8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Catalogue No.

EU2548

Alias

8-OHdG(8-Hydroxydeoxyguanosine)

Detection method

Competitive ELISA Coated with Antigen

Application

This immunoassay kit allows for the in vitro quantitative determination of 8-OHdG concentrations in serum plasma and other biological fluids.

Size

96T

Range

1.563-100ng/ml

Sensitivity

< 0.938ng/ml

Storage

4 €žÆ’ for 6 months

Recovery

Matrices listed below were spiked with certain level of 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 95
EDTA plasma(n=5) 89-102 95
UFH plasma(n=5) 85-101 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 86-102% 85-102% 85-102%
EDTA plasma(n=5) 84-101% 84-96% 82-101% 84-99%
UFH plasma(n=5) 81-98% 81-93% 80-86% 86-91%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
General
ELISA Type:
Competitive
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