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5-HIAA(5-Hydroxyindoleacetic Acid)ELISA Kit (UNES00048)

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SKU:
UNES00048
$567

Description

5-HIAA(5-Hydroxyindoleacetic Acid)ELISA Kit

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 5-HIAA. During the reaction, 5-HIAA in the sample or standard competes with a fixed amount of  5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  5-HIAA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of   5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve.

5-HIAA(5-Hydroxyindoleacetic Acid)ELISA Kit Information

Target Name:5-HIAA
Target Synonym:5-Hydroxyindoleacetic Acid
UNIProt ID:
Target Species:Universal
Size:96T
Type:Competitive
Detection Type:TMB
Detection Range:1.563--100ng/mL
Sensitivity:0.938ng/mL
Tested Sample Types:Serum, Plasma, Cell supernatant
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Additional Information

Reactivity:
Universal
ELISA Type:
Competitive
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