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3-NT / 3-Nitrotyrosine ELISA Kit

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SKU:
UNFI0036

Description

3-NT / 3-Nitrotyrosine ELISA Kit - Information

The 3-NT / 3-Nitrotyrosine ELISA Kit can assay for 3-NT / 3-Nitrotyrosine in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our 3-NT / 3-Nitrotyrosine ELISA Kits Work?

The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT / 3-Nitrotyrosine . During the reaction, 3-NT / 3-Nitrotyrosine in the sample or standard competes with a fixed amount of 3-NT / 3-Nitrotyrosine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 3-NT / 3-Nitrotyrosine . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-NT / 3-Nitrotyrosine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

3-NT / 3-Nitrotyrosine ELISA Kit - Data

Product name

3-NT (3-Nitrotyrosine) ELISA Kit

Catalogue No.

EU2560

Alias

3-NT(3-Nitrotyrosine)

Detection method

Competitive ELISA Coated with Antigen

Application

This immunoassay kit allows for the in vitro quantitative determination of 3-NT concentrations in serum plasma and other biological fluids.

Size

96T

Range

1.563-100ng/ml

Sensitivity

< 0.938ng/ml

Storage

4 €žÆ’ for 6 months

Recovery

Matrices listed below were spiked with certain level of 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-100 96
EDTA plasma(n=5) 85-105 98
UFH plasma(n=5) 87-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 86-105% 88-101% 88-104%
EDTA plasma(n=5) 83-96% 84-99% 84-99% 83-95%
UFH plasma(n=5) 81-95% 85-100% 90-98% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
General
ELISA Type:
Competitive
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