3-NT / 3-Nitrotyrosine ELISA Kit
3-NT / 3-Nitrotyrosine ELISA Kit - Information
The 3-NT / 3-Nitrotyrosine ELISA Kit can assay for 3-NT / 3-Nitrotyrosine in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.How our 3-NT / 3-Nitrotyrosine ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT / 3-Nitrotyrosine . During the reaction, 3-NT / 3-Nitrotyrosine in the sample or standard competes with a fixed amount of 3-NT / 3-Nitrotyrosine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 3-NT / 3-Nitrotyrosine . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-NT / 3-Nitrotyrosine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
3-NT / 3-Nitrotyrosine ELISA Kit - Data
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3-NT (3-Nitrotyrosine) ELISA Kit
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Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of 3-NT concentrations in serum plasma and other biological fluids.
4 Æ for 6 months
Matrices listed below were spiked with certain level of 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
For Research Use Only