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25-HVD3 / 25-Hydroxy Vitamin D3 ELISA Kit

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SKU:
UNFI0025

Description

25-HVD3 / 25-Hydroxy Vitamin D3 ELISA Kit - Information

The 25-HVD3 / 25-Hydroxy Vitamin D3 ELISA Kit can assay for 25-HVD3 / 25-Hydroxy Vitamin D3 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our 25-HVD3 / 25-Hydroxy Vitamin D3 ELISA Kits Work?

The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 25-HVD3 / 25-Hydroxy Vitamin D3. During the reaction, 25-HVD3 / 25-Hydroxy Vitamin D3 in the sample or standard competes with a fixed amount of 25-HVD3 / 25-Hydroxy Vitamin D3 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 25-HVD3 / 25-Hydroxy Vitamin D3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 25-HVD3 / 25-Hydroxy Vitamin D3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

25-HVD3 / 25-Hydroxy Vitamin D3 ELISA Kit - Data

Product name

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit

Catalogue No.

EU2544

Alias

25-HVD3(25-Hydroxy Vitamin D3)

Detection method

Competitive ELISA Coated with Antigen

Application

This immunoassay kit allows for the in vitro quantitative determination of 25-HVD3 concentrations in serum plasma and other biological fluids.

Size

96T

Range

3.125-200ng/ml

Sensitivity

< 1.875ng/ml

Storage

4 €žÆ’ for 6 months

Recovery

Matrices listed below were spiked with certain level of 25-HVD3 and the recovery rates were calculated by comparing the measured value to the expected amount of 25-HVD3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-102 96
EDTA plasma(n=5) 86-100 94
UFH plasma(n=5) 86-105 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-HVD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 85-100% 86-103% 86-100%
EDTA plasma(n=5) 88-99% 89-99% 86-98% 87-99%
UFH plasma(n=5) 80-91% 85-97% 81-97% 88-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
General
ELISA Type:
Competitive
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